TY - JOUR
T1 - Development of a 31P magnetic resonance spectroscopy technique to quantify NADH and NAD+ at 3 T
AU - Mevenkamp, Julian
AU - Bruls, Yvonne M.H.
AU - Mancilla, Rodrigo
AU - Grevendonk, Lotte
AU - Wildberger, Joachim E.
AU - Brouwers, Kim
AU - Hesselink, Matthijs K.C.
AU - Schrauwen, Patrick
AU - Hoeks, Joris
AU - Houtkooper, Riekelt H.
AU - Buitinga, Mijke
AU - de Graaf, Robin A.
AU - Lindeboom, Lucas
AU - Schrauwen-Hinderling, Vera B.
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2024/12/1
Y1 - 2024/12/1
N2 - NADH and NAD+ act as electron donors and acceptors and NAD+ was shown to stimulate mitochondrial biogenesis and metabolic health. We here develop a non-invasive Phosphorous Magnetic Resonance Spectroscopy (31P-MRS) method to quantify these metabolites in human skeletal muscle on a clinical 3 T MRI scanner. This new MR-sequence enables NADH and NAD+ quantification by suppressing α-ATP signal, normally overlapping with NADH and NAD+. The sequence is based on a double spin echo in combination with a modified z-Filter achieving strong α-ATP suppression with little effect on NAD+ and NADH. Here we test and validate it in phantoms and in humans by measuring reproducibility and detecting a physiological decrease in NAD+ and increase in NADH induced by ischemia. Furthermore, the 31P-MRS outcomes are compared to analysis in biopsies. Additionally, we show higher NAD+ and lower NADH content in physically active older adults compared to sedentary individuals, reflecting increased metabolic health.
AB - NADH and NAD+ act as electron donors and acceptors and NAD+ was shown to stimulate mitochondrial biogenesis and metabolic health. We here develop a non-invasive Phosphorous Magnetic Resonance Spectroscopy (31P-MRS) method to quantify these metabolites in human skeletal muscle on a clinical 3 T MRI scanner. This new MR-sequence enables NADH and NAD+ quantification by suppressing α-ATP signal, normally overlapping with NADH and NAD+. The sequence is based on a double spin echo in combination with a modified z-Filter achieving strong α-ATP suppression with little effect on NAD+ and NADH. Here we test and validate it in phantoms and in humans by measuring reproducibility and detecting a physiological decrease in NAD+ and increase in NADH induced by ischemia. Furthermore, the 31P-MRS outcomes are compared to analysis in biopsies. Additionally, we show higher NAD+ and lower NADH content in physically active older adults compared to sedentary individuals, reflecting increased metabolic health.
UR - https://www.scopus.com/pages/publications/85207388806
U2 - 10.1038/s41467-024-53292-4
DO - 10.1038/s41467-024-53292-4
M3 - Artículo
C2 - 39443469
AN - SCOPUS:85207388806
SN - 2041-1723
VL - 15
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 9159
ER -