In house validation for the direct determination of 5-hydroxymethyl-2-furfural (HMF) in “dulce de leche”

Josefina Barrera; Franco Pedreschi; Juan Pablo Gómez; Rommy N. Zúñiga; María Salomé Mariotti-Celis

doi:10.1016/j.jfca.2020.103665

In house validation for the direct determination of 5-hydroxymethyl-2-furfural (HMF) in “dulce de leche”

Josefina Barrera, Franco Pedreschi, Juan Pablo Gómez, Rommy N. Zúñiga, María Salomé Mariotti-Celis

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

In this work an HPLC-DAD determination method for free and total HMF in “dulce de leche” was developed and validated. The separation was carried out using a mobile phase of acetonitrile-water (4.5:95.5, v/v) on Acclaim™120 C18 (4.6 × 150 mm, 5 μm), at 30 °C and a flow rate of 1 mL/min. The detection was done at 284 nm.The method showed good selectivity and linearity (determination coefficient over 0.999). Low LOD and LOQ values were obtained (19.51 μg/L and 65.05 μg/L respectively). Relative standard deviation (RSD) for repeatability and intermediate precision was <4.12 % and <5.98 % respectively. Recovery rates were between 89.04 % and 91.89 %. In addition, the HMF contents in commercial “dulce de leche” from Chile was reported, ranging from 2.33 to 10.31 mg/kg for free HMF and 314.61–464.98 mg/kg for total HMF.

Original language	English
Article number	103665
Journal	Journal of Food Composition and Analysis
Volume	95
DOIs	https://doi.org/10.1016/j.jfca.2020.103665
State	Published - 1 Jan 2021

Keywords

5-hydroxymethyl-2-furfural
Dairy product
Food analysis
Food composition
HMF
HPLC-DAD
Validation
“dulce de leche”

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@article{dd83976813fc4146a267cb6454128e36,

title = "In house validation for the direct determination of 5-hydroxymethyl-2-furfural (HMF) in “dulce de leche”",

abstract = "In this work an HPLC-DAD determination method for free and total HMF in “dulce de leche” was developed and validated. The separation was carried out using a mobile phase of acetonitrile-water (4.5:95.5, v/v) on Acclaim{\texttrademark}120 C18 (4.6 × 150 mm, 5 μm), at 30 °C and a flow rate of 1 mL/min. The detection was done at 284 nm.The method showed good selectivity and linearity (determination coefficient over 0.999). Low LOD and LOQ values were obtained (19.51 μg/L and 65.05 μg/L respectively). Relative standard deviation (RSD) for repeatability and intermediate precision was <4.12 \% and <5.98 \% respectively. Recovery rates were between 89.04 \% and 91.89 \%. In addition, the HMF contents in commercial “dulce de leche” from Chile was reported, ranging from 2.33 to 10.31 mg/kg for free HMF and 314.61–464.98 mg/kg for total HMF.",

keywords = "5-hydroxymethyl-2-furfural, Dairy product, Food analysis, Food composition, HMF, HPLC-DAD, Validation, “dulce de leche”",

author = "Josefina Barrera and Franco Pedreschi and G{\'o}mez, \{Juan Pablo\} and Z{\'u}{\~n}iga, \{Rommy N.\} and Mariotti-Celis, \{Mar{\'i}a Salom{\'e}\}",

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T1 - In house validation for the direct determination of 5-hydroxymethyl-2-furfural (HMF) in “dulce de leche”

AU - Barrera, Josefina

AU - Pedreschi, Franco

AU - Gómez, Juan Pablo

AU - Zúñiga, Rommy N.

AU - Mariotti-Celis, María Salomé

PY - 2021/1/1

Y1 - 2021/1/1

N2 - In this work an HPLC-DAD determination method for free and total HMF in “dulce de leche” was developed and validated. The separation was carried out using a mobile phase of acetonitrile-water (4.5:95.5, v/v) on Acclaim™120 C18 (4.6 × 150 mm, 5 μm), at 30 °C and a flow rate of 1 mL/min. The detection was done at 284 nm.The method showed good selectivity and linearity (determination coefficient over 0.999). Low LOD and LOQ values were obtained (19.51 μg/L and 65.05 μg/L respectively). Relative standard deviation (RSD) for repeatability and intermediate precision was <4.12 % and <5.98 % respectively. Recovery rates were between 89.04 % and 91.89 %. In addition, the HMF contents in commercial “dulce de leche” from Chile was reported, ranging from 2.33 to 10.31 mg/kg for free HMF and 314.61–464.98 mg/kg for total HMF.

AB - In this work an HPLC-DAD determination method for free and total HMF in “dulce de leche” was developed and validated. The separation was carried out using a mobile phase of acetonitrile-water (4.5:95.5, v/v) on Acclaim™120 C18 (4.6 × 150 mm, 5 μm), at 30 °C and a flow rate of 1 mL/min. The detection was done at 284 nm.The method showed good selectivity and linearity (determination coefficient over 0.999). Low LOD and LOQ values were obtained (19.51 μg/L and 65.05 μg/L respectively). Relative standard deviation (RSD) for repeatability and intermediate precision was <4.12 % and <5.98 % respectively. Recovery rates were between 89.04 % and 91.89 %. In addition, the HMF contents in commercial “dulce de leche” from Chile was reported, ranging from 2.33 to 10.31 mg/kg for free HMF and 314.61–464.98 mg/kg for total HMF.

KW - 5-hydroxymethyl-2-furfural

KW - Dairy product

KW - Food analysis

KW - Food composition

KW - HMF

KW - HPLC-DAD

KW - Validation

KW - “dulce de leche”

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DO - 10.1016/j.jfca.2020.103665

M3 - Artículo

AN - SCOPUS:85091943585

SN - 0889-1575

VL - 95

JO - Journal of Food Composition and Analysis

JF - Journal of Food Composition and Analysis

M1 - 103665

ER -

In house validation for the direct determination of 5-hydroxymethyl-2-furfural (HMF) in “dulce de leche”

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Keywords

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