Protocol to measure spontaneous locomotion, graded exercise oxygen consumption, and cross-sectional area of skeletal muscle cells in zebrafish

Mauricio Castro-Sepulveda; Cassandra Tabasso; Dogan Grepper; Adrien Martinotti; Axel K.F. Aguettaz; Sylviane Lagarrigue; Francesca Amati

doi:10.1016/j.xpro.2025.103863

Protocol to measure spontaneous locomotion, graded exercise oxygen consumption, and cross-sectional area of skeletal muscle cells in zebrafish

Mauricio Castro-Sepulveda, Cassandra Tabasso, Dogan Grepper, Adrien Martinotti, Axel K.F. Aguettaz, Sylviane Lagarrigue, Francesca Amati

University of Lausanne

Research output: Contribution to journal › Article › peer-review

Abstract

Skeletal muscle function and quality are strong indicators of metabolic health. Here, we present a protocol for skeletal muscle phenotyping in zebrafish. We describe steps to evaluate spontaneous locomotion, measure oxygen consumption during incremental exercise, and analyze cross-sectional area of skeletal muscle cells in adult zebrafish. This protocol has potential applications to assess zebrafish muscle quality and function in studies of metabolic diseases, aging, and skeletal muscle health. For complete details on the use and execution of this protocol, please refer to Grepper and Tabasso et al.¹

Original language	English
Article number	103863
Journal	STAR Protocols
Volume	6
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2025.103863
State	Published - 20 Jun 2025
Externally published	Yes

Keywords

Metabolism
Model Organisms

Access to Document

10.1016/j.xpro.2025.103863

Cite this

@article{40e78d4b7e4440d0b4a79053dd006768,

title = "Protocol to measure spontaneous locomotion, graded exercise oxygen consumption, and cross-sectional area of skeletal muscle cells in zebrafish",

abstract = "Skeletal muscle function and quality are strong indicators of metabolic health. Here, we present a protocol for skeletal muscle phenotyping in zebrafish. We describe steps to evaluate spontaneous locomotion, measure oxygen consumption during incremental exercise, and analyze cross-sectional area of skeletal muscle cells in adult zebrafish. This protocol has potential applications to assess zebrafish muscle quality and function in studies of metabolic diseases, aging, and skeletal muscle health. For complete details on the use and execution of this protocol, please refer to Grepper and Tabasso et al.1",

keywords = "Metabolism, Model Organisms",

author = "Mauricio Castro-Sepulveda and Cassandra Tabasso and Dogan Grepper and Adrien Martinotti and Aguettaz, \{Axel K.F.\} and Sylviane Lagarrigue and Francesca Amati",

note = "Publisher Copyright: {\textcopyright} 2025 The Author(s)",

year = "2025",

month = jun,

day = "20",

doi = "10.1016/j.xpro.2025.103863",

language = "Ingl{\'e}s",

volume = "6",

journal = "STAR Protocols",

issn = "2666-1667",

publisher = "Cell Press",

number = "2",

}

TY - JOUR

T1 - Protocol to measure spontaneous locomotion, graded exercise oxygen consumption, and cross-sectional area of skeletal muscle cells in zebrafish

AU - Castro-Sepulveda, Mauricio

AU - Tabasso, Cassandra

AU - Grepper, Dogan

AU - Martinotti, Adrien

AU - Aguettaz, Axel K.F.

AU - Lagarrigue, Sylviane

AU - Amati, Francesca

PY - 2025/6/20

Y1 - 2025/6/20

N2 - Skeletal muscle function and quality are strong indicators of metabolic health. Here, we present a protocol for skeletal muscle phenotyping in zebrafish. We describe steps to evaluate spontaneous locomotion, measure oxygen consumption during incremental exercise, and analyze cross-sectional area of skeletal muscle cells in adult zebrafish. This protocol has potential applications to assess zebrafish muscle quality and function in studies of metabolic diseases, aging, and skeletal muscle health. For complete details on the use and execution of this protocol, please refer to Grepper and Tabasso et al.1

AB - Skeletal muscle function and quality are strong indicators of metabolic health. Here, we present a protocol for skeletal muscle phenotyping in zebrafish. We describe steps to evaluate spontaneous locomotion, measure oxygen consumption during incremental exercise, and analyze cross-sectional area of skeletal muscle cells in adult zebrafish. This protocol has potential applications to assess zebrafish muscle quality and function in studies of metabolic diseases, aging, and skeletal muscle health. For complete details on the use and execution of this protocol, please refer to Grepper and Tabasso et al.1

KW - Metabolism

KW - Model Organisms

UR - http://www.scopus.com/inward/record.url?scp=105007049470&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2025.103863

DO - 10.1016/j.xpro.2025.103863

M3 - Artículo

AN - SCOPUS:105007049470

SN - 2666-1667

VL - 6

JO - STAR Protocols

JF - STAR Protocols

IS - 2

M1 - 103863

ER -

Protocol to measure spontaneous locomotion, graded exercise oxygen consumption, and cross-sectional area of skeletal muscle cells in zebrafish

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this