Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography

Ciria G. Figueroa-Soto; Ana Maria Calderón De La Barca; Luz Vazquez-Moreno; Inocencio Higuera-Ciapara; Gloria Yepiz-Plascencia

doi:10.1016/S0305-0491(96)00321-5

Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography

Ciria G. Figueroa-Soto, Ana Maria Calderón De La Barca, Luz Vazquez-Moreno, Inocencio Higuera-Ciapara, Gloria Yepiz-Plascencia

Centro de Investigacion en Alimentacion y Desarrollo

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25 Scopus citations

Abstract

Hemocyanin (Hc) was isolated from white shrimp (Penaeus vannamei Boone) plasma by density gradient ultracentrifugation and immobilized metal affinity chromatography. Hc was contained in the subnatant high density fraction and was adsorbed by an iminodiacetic acid (Ni-DA) column that bound Hc and apohemocyanin. Hc molecular weight was estimated by pore limiting electrophoresis as 400 kDa. It is composed of two subunits of approximately 75 and 82 kDa. This 400-kDa protein contained copper and was detected by antibodies raised against the purified subunitsl Isoelectric points of the affinity purified native protein were 4.8 and 4.9. Presence of covalently bound carbohydrates in both subunits was detected by biotinylated lectins and avidinhorseradish peroxidase.

Original language	English
Pages (from-to)	203-208
Number of pages	6
Journal	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume	117
Issue number	2
DOIs	https://doi.org/10.1016/S0305-0491(96)00321-5
State	Published - 1 Jan 1997
Externally published	Yes

Keywords

Affinity
Antibodies
Copper
Crustacea
Hemolymph
Metal affinity
Shrimp hemocyanin

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10.1016/S0305-0491(96)00321-5

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Figueroa-Soto, C. G., De La Barca, A. M. C., Vazquez-Moreno, L., Higuera-Ciapara, I., & Yepiz-Plascencia, G. (1997). Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 117(2), 203-208. https://doi.org/10.1016/S0305-0491(96)00321-5

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title = "Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography",

abstract = "Hemocyanin (Hc) was isolated from white shrimp (Penaeus vannamei Boone) plasma by density gradient ultracentrifugation and immobilized metal affinity chromatography. Hc was contained in the subnatant high density fraction and was adsorbed by an iminodiacetic acid (Ni-DA) column that bound Hc and apohemocyanin. Hc molecular weight was estimated by pore limiting electrophoresis as 400 kDa. It is composed of two subunits of approximately 75 and 82 kDa. This 400-kDa protein contained copper and was detected by antibodies raised against the purified subunitsl Isoelectric points of the affinity purified native protein were 4.8 and 4.9. Presence of covalently bound carbohydrates in both subunits was detected by biotinylated lectins and avidinhorseradish peroxidase.",

keywords = "Affinity, Antibodies, Copper, Crustacea, Hemolymph, Metal affinity, Shrimp hemocyanin",

author = "Figueroa-Soto, \{Ciria G.\} and \{De La Barca\}, \{Ana Maria Calder{\'o}n\} and Luz Vazquez-Moreno and Inocencio Higuera-Ciapara and Gloria Yepiz-Plascencia",

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doi = "10.1016/S0305-0491(96)00321-5",

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Figueroa-Soto, CG, De La Barca, AMC, Vazquez-Moreno, L, Higuera-Ciapara, I & Yepiz-Plascencia, G 1997, 'Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography', Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, vol. 117, no. 2, pp. 203-208. https://doi.org/10.1016/S0305-0491(96)00321-5

Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography. / Figueroa-Soto, Ciria G.; De La Barca, Ana Maria Calderón; Vazquez-Moreno, Luz et al.
In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 117, No. 2, 01.01.1997, p. 203-208.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography

AU - Figueroa-Soto, Ciria G.

AU - De La Barca, Ana Maria Calderón

AU - Vazquez-Moreno, Luz

AU - Higuera-Ciapara, Inocencio

AU - Yepiz-Plascencia, Gloria

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Hemocyanin (Hc) was isolated from white shrimp (Penaeus vannamei Boone) plasma by density gradient ultracentrifugation and immobilized metal affinity chromatography. Hc was contained in the subnatant high density fraction and was adsorbed by an iminodiacetic acid (Ni-DA) column that bound Hc and apohemocyanin. Hc molecular weight was estimated by pore limiting electrophoresis as 400 kDa. It is composed of two subunits of approximately 75 and 82 kDa. This 400-kDa protein contained copper and was detected by antibodies raised against the purified subunitsl Isoelectric points of the affinity purified native protein were 4.8 and 4.9. Presence of covalently bound carbohydrates in both subunits was detected by biotinylated lectins and avidinhorseradish peroxidase.

AB - Hemocyanin (Hc) was isolated from white shrimp (Penaeus vannamei Boone) plasma by density gradient ultracentrifugation and immobilized metal affinity chromatography. Hc was contained in the subnatant high density fraction and was adsorbed by an iminodiacetic acid (Ni-DA) column that bound Hc and apohemocyanin. Hc molecular weight was estimated by pore limiting electrophoresis as 400 kDa. It is composed of two subunits of approximately 75 and 82 kDa. This 400-kDa protein contained copper and was detected by antibodies raised against the purified subunitsl Isoelectric points of the affinity purified native protein were 4.8 and 4.9. Presence of covalently bound carbohydrates in both subunits was detected by biotinylated lectins and avidinhorseradish peroxidase.

KW - Affinity

KW - Antibodies

KW - Copper

KW - Crustacea

KW - Hemolymph

KW - Metal affinity

KW - Shrimp hemocyanin

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U2 - 10.1016/S0305-0491(96)00321-5

DO - 10.1016/S0305-0491(96)00321-5

M3 - Artículo

AN - SCOPUS:0030805082

SN - 0305-0491

VL - 117

SP - 203

EP - 208

JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

IS - 2

ER -

Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography

Abstract

Keywords

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