TY - JOUR
T1 - Sustainable Recovery of Phlorotannins from Durvillaea incurvata
T2 - Integrated Extraction and Purification with Advanced Characterization
AU - Rivera-Tovar, Pamela Raquel
AU - Contreras-Contreras, Gabriela
AU - Rivas-Reyes, Paulina Isabel
AU - Pérez-Jiménez, Jara
AU - Martínez-Cifuentes, Maximiliano
AU - Pérez-Correa, José Ricardo
AU - Mariotti-Celis, María Salomé
N1 - Publisher Copyright:
© 2025 by the authors.
PY - 2025/3/1
Y1 - 2025/3/1
N2 - The rising demand for bioactive compounds from marine resources highlights the need for sustainable separation technologies. This study introduces an integrated process combining ultrasound-assisted extraction (USAE) and resin purification (RP) to isolate phlorotannins from Durvillaea incurvata, a brown seaweed with significant biomedical potential. Using a 32.5% ethanol–water solvent system for USAE followed by RP on Diaion HP-20 resin, phlorotannins were enriched 2.4-fold, with simultaneous removal of interfering compounds such as mannitol (~100%), which was demonstrated by FTIR and HPLC-IR analysis. Advanced characterization using UHPLC-QToF-MS/MS identified five novel phlorotannins with polymerization degrees of 3 to 8 phloroglucinol units in both USAE extracts and post-RP. Mass balance based on spectrophotometric measurements indicated a purification factor of ~2, confirming process effectiveness. RP streams showed distinct phlorotannin profiles, with one phlorotannin exceeding 70% relative abundance. However, MS/MS results showed significantly lower recoveries than spectrophotometric data, revealing a novel insight into RP purification. These findings highlight the critical role of comprehensive chemical characterization in optimizing sustainable phlorotannin extraction from seaweed. They propose a framework for scalable, eco-efficient technologies for achieving high-purity phlorotannin recovery. This approach facilitates the development of phlorotannin-based applications in the nutraceutical and pharmaceutical industries.
AB - The rising demand for bioactive compounds from marine resources highlights the need for sustainable separation technologies. This study introduces an integrated process combining ultrasound-assisted extraction (USAE) and resin purification (RP) to isolate phlorotannins from Durvillaea incurvata, a brown seaweed with significant biomedical potential. Using a 32.5% ethanol–water solvent system for USAE followed by RP on Diaion HP-20 resin, phlorotannins were enriched 2.4-fold, with simultaneous removal of interfering compounds such as mannitol (~100%), which was demonstrated by FTIR and HPLC-IR analysis. Advanced characterization using UHPLC-QToF-MS/MS identified five novel phlorotannins with polymerization degrees of 3 to 8 phloroglucinol units in both USAE extracts and post-RP. Mass balance based on spectrophotometric measurements indicated a purification factor of ~2, confirming process effectiveness. RP streams showed distinct phlorotannin profiles, with one phlorotannin exceeding 70% relative abundance. However, MS/MS results showed significantly lower recoveries than spectrophotometric data, revealing a novel insight into RP purification. These findings highlight the critical role of comprehensive chemical characterization in optimizing sustainable phlorotannin extraction from seaweed. They propose a framework for scalable, eco-efficient technologies for achieving high-purity phlorotannin recovery. This approach facilitates the development of phlorotannin-based applications in the nutraceutical and pharmaceutical industries.
KW - brown seaweed
KW - integrated extraction–purification processes
KW - mass balance
KW - spectrophotometric
KW - UHPLC-QToF MS/MS
UR - http://www.scopus.com/inward/record.url?scp=105001372013&partnerID=8YFLogxK
U2 - 10.3390/antiox14030250
DO - 10.3390/antiox14030250
M3 - Artículo
AN - SCOPUS:105001372013
SN - 2076-3921
VL - 14
JO - Antioxidants
JF - Antioxidants
IS - 3
M1 - 250
ER -