Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography

Ciria G. Figueroa-Soto; Ana Maria Calderón De La Barca; Luz Vazquez-Moreno; Inocencio Higuera-Ciapara; Gloria Yepiz-Plascencia

doi:10.1016/S0305-0491(96)00321-5

Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography

Ciria G. Figueroa-Soto
, Ana Maria Calderón De La Barca
, Luz Vazquez-Moreno
, Inocencio Higuera-Ciapara
, Gloria Yepiz-Plascencia^*

^*Autore corrispondente per questo lavoro

Centro de Investigacion en Alimentacion y Desarrollo

Risultato della ricerca › peer review

25 Citazioni (Scopus)

Abstract

Hemocyanin (Hc) was isolated from white shrimp (Penaeus vannamei Boone) plasma by density gradient ultracentrifugation and immobilized metal affinity chromatography. Hc was contained in the subnatant high density fraction and was adsorbed by an iminodiacetic acid (Ni-DA) column that bound Hc and apohemocyanin. Hc molecular weight was estimated by pore limiting electrophoresis as 400 kDa. It is composed of two subunits of approximately 75 and 82 kDa. This 400-kDa protein contained copper and was detected by antibodies raised against the purified subunitsl Isoelectric points of the affinity purified native protein were 4.8 and 4.9. Presence of covalently bound carbohydrates in both subunits was detected by biotinylated lectins and avidinhorseradish peroxidase.

Lingua originale	English
pagine (da-a)	203-208
Numero di pagine	6
Rivista	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume	117
Numero di pubblicazione	2
DOI	https://doi.org/10.1016/S0305-0491(96)00321-5
Stato di pubblicazione	Published - 1 gen 1997
Pubblicato esternamente	Sì

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10.1016/S0305-0491(96)00321-5

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https://www.scopus.com/pages/publications/0030805082

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Figueroa-Soto, C. G., De La Barca, A. M. C., Vazquez-Moreno, L., Higuera-Ciapara, I., & Yepiz-Plascencia, G. (1997). Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 117(2), 203-208. https://doi.org/10.1016/S0305-0491(96)00321-5

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title = "Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography",

abstract = "Hemocyanin (Hc) was isolated from white shrimp (Penaeus vannamei Boone) plasma by density gradient ultracentrifugation and immobilized metal affinity chromatography. Hc was contained in the subnatant high density fraction and was adsorbed by an iminodiacetic acid (Ni-DA) column that bound Hc and apohemocyanin. Hc molecular weight was estimated by pore limiting electrophoresis as 400 kDa. It is composed of two subunits of approximately 75 and 82 kDa. This 400-kDa protein contained copper and was detected by antibodies raised against the purified subunitsl Isoelectric points of the affinity purified native protein were 4.8 and 4.9. Presence of covalently bound carbohydrates in both subunits was detected by biotinylated lectins and avidinhorseradish peroxidase.",

keywords = "Affinity, Antibodies, Copper, Crustacea, Hemolymph, Metal affinity, Shrimp hemocyanin",

author = "Figueroa-Soto, \{Ciria G.\} and \{De La Barca\}, \{Ana Maria Calder{\'o}n\} and Luz Vazquez-Moreno and Inocencio Higuera-Ciapara and Gloria Yepiz-Plascencia",

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doi = "10.1016/S0305-0491(96)00321-5",

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Figueroa-Soto, CG, De La Barca, AMC, Vazquez-Moreno, L, Higuera-Ciapara, I & Yepiz-Plascencia, G 1997, 'Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography', Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, vol. 117, n. 2, pagg. 203-208. https://doi.org/10.1016/S0305-0491(96)00321-5

TY - JOUR

T1 - Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography

AU - Figueroa-Soto, Ciria G.

AU - De La Barca, Ana Maria Calderón

AU - Vazquez-Moreno, Luz

AU - Higuera-Ciapara, Inocencio

AU - Yepiz-Plascencia, Gloria

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Hemocyanin (Hc) was isolated from white shrimp (Penaeus vannamei Boone) plasma by density gradient ultracentrifugation and immobilized metal affinity chromatography. Hc was contained in the subnatant high density fraction and was adsorbed by an iminodiacetic acid (Ni-DA) column that bound Hc and apohemocyanin. Hc molecular weight was estimated by pore limiting electrophoresis as 400 kDa. It is composed of two subunits of approximately 75 and 82 kDa. This 400-kDa protein contained copper and was detected by antibodies raised against the purified subunitsl Isoelectric points of the affinity purified native protein were 4.8 and 4.9. Presence of covalently bound carbohydrates in both subunits was detected by biotinylated lectins and avidinhorseradish peroxidase.

AB - Hemocyanin (Hc) was isolated from white shrimp (Penaeus vannamei Boone) plasma by density gradient ultracentrifugation and immobilized metal affinity chromatography. Hc was contained in the subnatant high density fraction and was adsorbed by an iminodiacetic acid (Ni-DA) column that bound Hc and apohemocyanin. Hc molecular weight was estimated by pore limiting electrophoresis as 400 kDa. It is composed of two subunits of approximately 75 and 82 kDa. This 400-kDa protein contained copper and was detected by antibodies raised against the purified subunitsl Isoelectric points of the affinity purified native protein were 4.8 and 4.9. Presence of covalently bound carbohydrates in both subunits was detected by biotinylated lectins and avidinhorseradish peroxidase.

KW - Affinity

KW - Antibodies

KW - Copper

KW - Crustacea

KW - Hemolymph

KW - Metal affinity

KW - Shrimp hemocyanin

UR - https://www.scopus.com/pages/publications/0030805082

U2 - 10.1016/S0305-0491(96)00321-5

DO - 10.1016/S0305-0491(96)00321-5

M3 - Artículo

AN - SCOPUS:0030805082

SN - 0305-0491

VL - 117

SP - 203

EP - 208

JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

IS - 2

ER -

Purification of hemocyanin from white shrimp (Penaeus vannamei boone) by immobilized metal affinity chromatography

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