Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons
- Kathia Luenebergb(Author),
- Guadalupe Domínguezb(Author),
- Oscar Arias-Carriánc(Author),
- Marcela Palomero-Riveroc(Author),
- Diana Millán-Aldacob(Author),
- Julio Moránb(Author)
- ,
- bUniversidad Nacional Autónoma de México,
- cUniversity of Marburg
Open access
Publication Information
Output type
Original language
EnglishArticle number
28Journal (Volume, Issue Number)
International Archives of Medicine (Volume 4, Issue 1)Publication milestones
- Published - 23/08/2011
Publication status
External Publication IDs
- Scopus: 80051781278
Abstract
The endocannabinoid anandamide (ANA) participates in the control of cell death inducing the formation of apoptotic bodies and DNA fragmentation. The aim of this study was to evaluate whether the ANA degrading enzyme, the fatty acid amide hydrolase (FAAH), would induce cellular death. Experiments were performed in cerebellar granule neurons cultured with the FAAH inhibitor, URB597 (25, 50 or 100 nM) as well as endogenous lipids such as oleoylethanolamide (OEA) or palmitoylethanolamide (PEA) and cellular viability was determined by MTT test. Neurons cultured with URB597 (25, 50 or 100 nM) displayed a decrease in cellular viability. In addition, if cultured with OEA (25 nM) or PEA (100 nM), cellular death was found. These results further suggest that URB597, OEA or PEA promote cellular death.