Sustainable Recovery of Phlorotannins from Durvillaea incurvata: Integrated Extraction and Purification with Advanced Characterization
- Pamela Raquel Rivera-Tovare(Author),
- Gabriela Contreras-Contrerase(Author),
- Paulina Isabel Rivas-Reyesg(Author),
- Jara Pérez-Jiménezi, j(Author),
- Maximiliano Martínez-Cifuentesf(Author),
- José Ricardo Pérez-Correah(Author)
- ,
- ,
- ,
- ,
- eUniversidad Finis Terrae,
- fUniversidad de Concepción
Open access
Publication Information
Output type
Original language
EnglishArticle number
250Journal (Volume, Issue Number)
Antioxidants (Volume 14, Issue 3)Publication milestones
- Published - 01/03/2025
Publication status
External Publication IDs
- Scopus: 105001372013
Abstract
The rising demand for bioactive compounds from marine resources highlights the need for sustainable separation technologies. This study introduces an integrated process combining ultrasound-assisted extraction (USAE) and resin purification (RP) to isolate phlorotannins from Durvillaea incurvata, a brown seaweed with significant biomedical potential. Using a 32.5% ethanol–water solvent system for USAE followed by RP on Diaion HP-20 resin, phlorotannins were enriched 2.4-fold, with simultaneous removal of interfering compounds such as mannitol (~100%), which was demonstrated by FTIR and HPLC-IR analysis. Advanced characterization using UHPLC-QToF-MS/MS identified five novel phlorotannins with polymerization degrees of 3 to 8 phloroglucinol units in both USAE extracts and post-RP. Mass balance based on spectrophotometric measurements indicated a purification factor of ~2, confirming process effectiveness. RP streams showed distinct phlorotannin profiles, with one phlorotannin exceeding 70% relative abundance. However, MS/MS results showed significantly lower recoveries than spectrophotometric data, revealing a novel insight into RP purification. These findings highlight the critical role of comprehensive chemical characterization in optimizing sustainable phlorotannin extraction from seaweed. They propose a framework for scalable, eco-efficient technologies for achieving high-purity phlorotannin recovery. This approach facilitates the development of phlorotannin-based applications in the nutraceutical and pharmaceutical industries.